Peroxisomal localization of Arabidopsis isopentenyl diphosphate isomerases suggests that part of the plant isoprenoid mevalonic acid pathway is compartmentalized to peroxisomes.

نویسندگان

  • Maya Sapir-Mir
  • Anahit Mett
  • Eduard Belausov
  • Shira Tal-Meshulam
  • Ahuva Frydman
  • David Gidoni
  • Yoram Eyal
چکیده

The electronic form of this issue, available as of November 11, 2008, at www.plantphysiol.org, is considered the journal of record. On the Cover: A confocal three-dimensional maximum intensity projection of Bacillus subtilis biofilm formation (green) on living Arabidopsis thaliana Col-0 root stained with Calcoflour White (blue). Bacteria and plant nuclei (green) stained green with vital nucleic acid stain Syto 13. The cover image was created and provided by Drs. Thimmaraju Rudrappa and Kirk Czymmek of the University of Delaware. (See Rudrappa et al., pp. 1547–1556.)

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Scientific Correspondence Peroxisomal Localization of Arabidopsis Isopentenyl Diphosphate Isomerases Suggests That Part of the Plant Isoprenoid Mevalonic Acid Pathway Is Compartmentalized to Peroxisomes

Isoprenoids, the largest family of natural products, play numerous vital roles in basic plant processes, including photosynthesis, growth and development, reproduction, plant defense, and adaptation to environmental conditions (Gershenzon and Kreis, 1999; Rodriguez-Concepcion and Boronat, 2002). All isoprenoids are synthesized via the condensation of the fivecarbon universal isoprenoid precurso...

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The Arabidopsis thaliana type I Isopentenyl Diphosphate Isomerases are targeted to multiple subcellular compartments and have overlapping functions in isoprenoid biosynthesis.

To form the building blocks of isoprenoids, isopentenyl diphosphate (IPP) isomerase activity, which converts IPP to dimethylallyl diphosphate (DMAPP), appears to be necessary in cytosol, plastids, and mitochondria. Arabidopsis thaliana contains only two IPP isomerases (Isopentenyl Diphosphate Isomerase1 [IDI1] and IDI2). Both encode proteins with N-terminal extensions similar to transit peptide...

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Dedicated roles of plastid transketolases during the early onset of isoprenoid biogenesis in pepper fruits1.

Isopentenyl diphosphate (IPP), which is produced from mevalonic acid or other nonmevalonic substrates, is the universal precursor of isoprenoids in nature. Despite the presence of several isoprenoid compounds in plastids, enzymes of the mevalonate pathway leading to IPP formation have never been isolated or identified to our knowledge. We now describe the characterization of two pepper (Capsicu...

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Farnesylation directs AtIPT3 subcellular localization and modulates cytokinin biosynthesis in Arabidopsis.

Cytokinins regulate cell division and differentiation as well as a number of other processes implicated in plant development. The first step of cytokinin biosynthesis in Arabidopsis (Arabidopsis thaliana) is catalyzed by adenosine phosphate-isopentenyltransferases (AtIPT). The enzymes are localized in plastids or the cytoplasm where they utilize the intermediate dimethylallyl-diphosphate from t...

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Isoprenoid biosynthesis in rat liver peroxisomes. Characterization of cis-prenyltransferase and squalene synthetase.

Isolated peroxisomes were able to utilize [3H]isopentenyl diphosphate to synthesize farnesyl diphosphate, which then was utilized as substrate by both the peroxisomal squalene synthetase and cis-prenyltransferase. The specific activity of squalene synthetase in peroxisomes was as high as in microsomes, i.e. 160 pmol/mg of protein/min. If NADPH was omitted from the assay medium, presqualene diph...

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عنوان ژورنال:
  • Plant physiology

دوره 148 3  شماره 

صفحات  -

تاریخ انتشار 2008